Lesson 1 – Separating Proteins using SDS Polyacrylamide Gel Electrophoresis
SDS Polyacrylamide Gel Electrophoresis is a technique that allows us to separate protein molecules by size. In this video tutorial, we show you how to perform electrophoresis of protein samples. The proteins have been denatured using Sodium Dodecyl Sulfate and reducing agents before electrophoresis, which allows us to accurately determine the size of the polypeptide chain.